Introduction to ultrapure water generator laboratory
The laboratory ultrapure water generator is a device used to produce high-purity water, with the main purpose of meeting the laboratory’s demand for ultrapure water. Ultra pure water usually refers to water with a resistivity close to the theoretical limit (18.3 M Ω· cm). It is very important in scientific experiments because any small impurities can affect the experimental results.
The working principle and composition of our laboratory’s ultrapure water generator mainly include the following stages:
Pre treatment stage: The main task of this stage is to remove large particulate pollutants, suspended solids, and partially soluble substances from tap water.
Desalination stage: Further removal of ion impurities in water through reverse osmosis (RO) technology. Reverse osmosis technology uses semi permeable membranes to separate salt and other soluble substances from water;
Polishing stage: This is the final purification step, using high-efficiency mixed bed ion exchange resin ASTM Type I standard.
Disinfection stage: UV disinfection is usually used, which can effectively remove endotoxins, DNases, RNases, and microbial contamination.
Parameter of deionizer water machine
| Model | DI-15 | DI-30 | DI-45 | DI-60 | |||
| Output(25℃)* | 15 liters/hour | 30 liters/hour | 45 liters/hour | 60 liters/hour | |||
| Pure water outlet | One deionized water and one reverse osmosis water | ||||||
| Deionized water quality | |||||||
| Resistivity | 15-18.2MΩ.cm | ||||||
| Particle(>0.1μm) | N/A | N/A | N/A | N/A | |||
| RO water quality | |||||||
| Ion rejection rate | 96%-99% (new RO membrane) | ||||||
| Organic rejection rate | >99%, when MW>200 Dalton | ||||||
| Particles rejection rate | >99% | ||||||
| Feed water requirements | Tap water, temperature:5-45℃,pressure:1.0-4.0Kgf/cm2 | ||||||
| Electrical requirements | AC110-240V, 50/60Hz | ||||||
| Power | 55W | 60W | 70W | 78W | |||
Application of
The application of ultrapure water in laboratories is very extensive, mainly reflected in the following aspects:
- Cell culture: Ultra pure water is an important component of cell culture. Due to its low electrical resistivity (usually 18.2 M Ω· cm) and low organic carbon content (less than 5 ppb). It can effectively avoid impurity contamination generated during cellular metabolism. In addition, the ultrapure water system is equipped with components such as activated carbon, ion exchange resin, and ultrafiltration. It can remove pollutants such as endotoxins, DNases, and RNases, ensuring high precision and reliability of cell culture.
- High performance liquid chromatography (HPLC): In HPLC analysis, ultrapure water is used for the preparation of the mobile phase to ensure the accuracy and reproducibility of the analysis results. The modular design of the ultrapure water system enables it to provide customized water quality parameters such as conductivity and TOC values according to different experimental needs. Gas chromatography-mass spectrometry (GC-MS): GC-MS analysis requires high-purity solvents and rinsing solutions. And ultrapure water can be used to prepare these reagents. Thereby improving the sensitivity and accuracy of analysis
. - Atomic Absorption Spectroscopy (AAS): AAS analysis requires extremely high water quality. And ultrapure water can be used for instrument cleaning and sample dilution to prevent sample contamination and improve the reliability of detection results
. - Electrophoresis: In electrophoresis experiments, ultrapure water is used for the preparation of buffer solutions and electrophoresis media to ensure stable conductivity during the electrophoresis process. Thereby obtaining high-quality electrophoresis results.
- Microbiological research: Ultra pure water systems can be used to produce high-purity microbial culture media and reagents. Through built-in ultraviolet lamps and pretreatment columns, microbial contamination can be effectively removed to ensure the reliability of experimental data.
- Other key biological applications, such as monoclonal antibody production, gene cloning, and protein expression, require the use of ultrapure water to ensure the quality and purity of the final product.